Gene Transfer Mechanism in Bacteria. Mechanism. The prototypical conjugative plasmid is the F-plasmid, or F-factor. Three broad mechanisms mediate efficient movement of DNA between cells- conjugation, transduction and transformation. The genetic material that is transferred through bacterial conjugation is a small plasmid, known as F-plasmid (F for fertility factor), that carries genetic information different from that which is already present in the chromosomes of the bacterial cell. Bacterial conjugation is a genetic transfer that involves cell‐to‐cell between donor and recipient cells. Bacterial conjugation involves the following steps: Pilus Formation. 7/23/2016 3 4. This tube formed between both cells (i.e., the donor and recipient bacterial cell) is known as sex pilus or just pilus. The donor cells (F+ cells) form a sex pilus and begin contact with an F- recipient cell. Conjugation is the process by which one bacterium transfers genetic material to another through direct contact. Bacterial conjugation is a way by which a bacterial cell transfers genetic material to another bacterial cell. Bacterial conjugation is the transfer of genetic material between bacterial cells by direct cell-to-cell contact or by a bridge-like connection between two cells. The F-plasmid is an episome (a plasmid that can integrate itself into the bacterial chromosome by homologous recombination) with a length of about 100 kb. Physical Contact between Donor and Recipient Cell. The T-DNA is covered by the VirE2 protein made by Agrobacterium and also, when it enters the plant cell, by the plant VIP1 protein. The pilus forms a conjugation tube and enables direct contact between the donor and the recipient cells. Bacterial Conjugation Definition. In conjugation, DNA is transferred between bacteria through a tube between cells. With the current method used to teach students in genetic courses at the undergraduate level, the transconjugants are identified using bacterial physiology and/or antibiotic resistance. Bacterial conjugation is a promiscuous DNA transport mechanism. Moreover, DNA can … MECHANISM OF CONJUGATION. Conjugative plasmids transfer themselves between most bacteria, thus being one of the main causal agents of the spread of antibiotic resistance among pathogenic bacteria. The mechanism resembles bacterial conjugation and the “virulence” genes of the Ti-plasmid are equivalent to the tra genes of other plasmids. Bacterial Conjugation - Mechanism. Mechanism of Bacterial Conjugation. Conjugation: Transfer of genes between cells that are in physical contact with one another; Transduction: Transfer of genes from one cell to another by a bacteriophage