Main Difference – Plasmid vs Vector. 8.4.1 Plasmids are Naturally Present in Some Bacteria. CRISPR Plasmids. The ease with which plasmids can be modified and replicated makes it a great tool in genetic engineering and biotechnology; For genetic engineering purpose, plasmids are artificially prepared in the lab Plasmid and vector are two types of double-stranded DNA molecules that have different functions in the cell. Expression vector: This type of vector/ plasmid is used to study the expression of a gene of our interest. For an example E.coli plasmid vector, should have following features: a. Ori site for replication. Figure 1. Supplement See also: plasmid; vector; genetic engineering. Plasmids are ubiquitous in prokaryotes but they have also been found in a number of eukaryotes, e.g. Commonly used fluorescent protein genes and vectors. Plasmid vector. Gateway® Cloning Vectors. Examples of available commercial plasmids approved for clinical use include pVAX1 and pcDNA3.1 vectors (Invitrogen, Thermo Fisher Scientific). Saccharomyces cerevisiae, which may contain a 2-micrometre-ring of plasmid. The main difference between plasmid and vectors is that plasmid is an extra-chromosomal element of mainly bacterial cells whereas vector is a vehicle that carries foreign DNA molecules into another cell. CRISPR plasmids for genome editing and gene regulation from Addgene, transOMIC, and others. Plasmid as Cloning Vector: 1. Hypothetical structure of a plasmid-based DNA vaccine encoding of A-B fusion protein. Plasmid are used as cloning vectors, include ori site or origin of replication, needed for replication in the bacterial cells. Plasmids and bacteriophages are frequently used as a cloning vector in the DNA recombinant technology. Fluorescent Protein Genes & Plasmids. For example if your recombinant plasmid contains a kanamycin cassette, then only the cells containing the plasmid will grow on an agar plate containing kanamycin. Commonly used Gateway® sequences including Donor Vectors, Entry Vectors, and Destination Vectors. Plasmids are extra chromosomal circular double stranded DNA, present in bacterial cells. Many bacteria contain extra-chromosomal DNA elements called plasmids.These are usually small (a few 1000 bp), circular, double stranded molecules that replicate independently of the chromosome and can be present in high copy numbers within a cell. b. Plasmids are important in certain bacteria since plasmids code for proteins, especially enzymes, which can confer resistance to antibiotics. Definition noun Plasmid manipulated by genetic engineering to serve as a tool to produce the desired products in the target cell through a biomolecular process such by the expression of gene of interest. Reporter plasmid: This type of plasmid is used to study the function of a gene. Plasmid Vector. Applications of plasmid DNA: As we explained in the above section, the plasmid DNA is used in the gene therapy to transfer gene of interest in specific cell type.