Recombinant DNA Technology. Recombinant DNA technology combines DNA from different sources to create a different sequence of DNA. Recombinant DNA is a molecule of DNA that has been modified to include genes from multiple sources, either through genetic recombination or through laboratory techniques. Recombinant DNA technology has been effectively used to produce various human proteins in microorganisms, such as insulin and growth hormone, used in the treatment of diseases (see Chapter 4: Recombinant DNA Technology … Selection of an appropriate cloning vector. The last step of recombinant DNA technology is aimed at increasing the production of the desired product. With the discovery of recombinant DNA technology, all that changed. Articulation and proliferation of DNA … Definition. Generally, recombinant DNA technology is used to increase copies of a given gene in … Insertion of DNA into vector to structure recombinant DNA molecule. In the lab, bacteria can be transformed with recombinant DNA. Selection of altered host cells. than in 1972, Berg, Cohen and Boyer met up for the discussion of a collaborative project so that after that they would be able to start up the process of recombinant DNA technology. by Microb Life. By the end of the 1970s only a few dozen genes had been mapped to autosomes because the number of usable markers was so few. for the purpose of cutting DNA endonuclease and restriction enzymes are used. By recombinant DNA … Steps of Recombinant DNA Technology: Selection and seclusion of DNA to be inserted. Recombinant DNA technology is used in a wide range of applications from vaccine production to the production of genetically engineered crops. recombinant DNA molecule is launched into a suitable host. Recombinant DNA Technology: Definition, Steps & Uses. As recombinant DNA technology …